Identification of the chemical components of ethanol extract of Chenopodium ambrosioides and evaluation of their in vitro antioxidant and anti tumor activities

Purpose: To determine the characteristic chemical components of ethanol extract Chenopodium ambrosioides and evaluate their antioxidant anti-tumor effects in vitro.
 Methods: The plant powder (5 g) was extracted with 1 L 80 % at room temperature for 45 min, then placed 60 oC varying microwave power duration to obtain optimal extraction conditions. Characteristic were detected using ultra-high performance liquid chromatography quadrupole time flight mass spectrometry (UPLC-Q-TOF-MS/MS). Kaempferitrin isolated from a D101 macroporous resin column, its content assessed by high (HPLC). effect kaempferitrin evaluated ability scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH) 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radicals, while anti-proliferation activity human liver cancer cells SMMC-7721 determined cell counting kit-8 (CCK-8) reagent.
 Results: Three C. obtained, namely, kaempferitrin, kaempferol-3-O-apigenin-7-O-rhamnoside kaempferol-3-O-acetylapigenin-7-O-rhamnoside. shown possess strong DPPH radical moderate ABTS scavenging activities. significantly inhibited proliferation doses 4 8 μg/mL, half-maximal concentration (IC50) 0.38 μM (p < 0.05).
 Conclusion: has results reported here indicate that may have potential use herbal medicine practice.